One of the central assumptions of genetic inheritance is that only a mutation in a germline cell (e.g., a sperm or egg cell) can be…
Individual StringTie Quants After aligning your RNA-seq samples to a reference genome with STAR (see our two-part STAR tutorial here and here), the alignment data…
I had the great privilege of attending the Intelligent Systems for Molecular Biology conference in Madison, July 10–14. Here, I’m sharing about something interesting I…
I had the great privilege of attending the Intelligent Systems for Molecular Biology conference in Madison, July 10–14. Here, I’m sharing about something interesting I…
From part 1 of this tutorial you should have created STAR indexes for your reference genome, such that you have a reference directory containing the…
In this multi-part tutorial we’ll walk step-by-step through a standard RNA alignment using the open-source tool STAR (Spliced Transcripts Alignment to a Reference). Alignment is the…
Before investing in a large and expensive RNA sequencing experiment, it’s a good idea to ensure your sample size is sufficient to obtain good statistical…
I had the great privilege of attending the Intelligent Systems for Molecular Biology conference in Madison, July 10–14. Here, I’m sharing about something interesting I…
A promising new method of targeting cancer cells takes advantage of the fact that the same mutation cascades that lead to cancer often damage the…