Individual StringTie Quants After aligning your RNA-seq samples to a reference genome with STAR (see our two-part STAR tutorial here and here), the alignment data…
From part 1 of this tutorial you should have created STAR indexes for your reference genome, such that you have a reference directory containing the…
In this multi-part tutorial we’ll walk step-by-step through a standard RNA alignment using the open-source tool STAR (Spliced Transcripts Alignment to a Reference). Alignment is the…
Before investing in a large and expensive RNA sequencing experiment, it’s a good idea to ensure your sample size is sufficient to obtain good statistical…